Anti-FGFR3 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-FGFR3 Rabbit Monoclonal Antibody catalog # M00200. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00200

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Fibroblast growth factor receptor 3; FGFR-3;2.7.10.1; CD333; FGFR3; JTK4

Gene Name

FGFR3

Gene ID

2261

UniProt

P22607

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human FGFR3

Clonality

Monoclonal

Clone

Clone: AAID-6

Tissue Specificity

Expressed in brain, kidney and testis. Very low or no expression in spleen, heart, and muscle. In 20- to 22- week old fetuses it is expressed at high level in kidney, lung, small intestine and brain, and to a lower degree in spleen, liver, and muscle. Isoform 2 is detected in epithelial cells. Isoform 1 is not detected in epithelial cells. Isoform 1 and isoform 2 are detected in fibroblastic cells. .

Applications

WB

Field of Research

Angiogenesis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Neural Stem Cells, Signal Transduction, Stem Cells

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. Plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. Regulates both osteogenesis and postnatal bone mineralization by osteoblasts. Promotes apoptosis in chondrocytes, but can also promote cancer cell proliferation. Required for normal development of the inner ear. Phosphorylates PLCG1, CBL and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Plays a role in the regulation of vitamin D metabolism. Mutations that lead to constitutive kinase activation or impair normal FGFR3 maturation, internalization and degradation lead to aberrant signaling. Over-expressed or constitutively activated FGFR3 promotes activation of PTPN11/SHP2, STAT1, STAT5A and STAT5B. Secreted isoform 3 retains its capacity to bind FGF1 and FGF2 and hence may interfere with FGF signaling. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

87710 MW

Observed Molecular Weight

103 kDa

Applications Notes

WB 1:500-1:2000

Gene Name Synonym

Fibroblast growth factor receptor 3

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle. Endoplasmic reticulum. The activated receptor is rapidly internalized and degraded. Detected in intracellular vesicles after internalization of the autophosphorylated receptor.

Protein Name

P2X purinoceptor 1

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.