Anti-RPE65 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-RPE65 Rabbit Monoclonal Antibody catalog # M00824. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00824

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Retinoid isomerohydrolase;3.1.1.64; All-trans-retinyl-palmitate hydrolase; Retinal pigment epithelium-specific 65 kDa protein; Retinol isomerase; RPE65

Gene Name

RPE65

Gene ID

6121

UniProt

Q16518

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human RPE65

Clonality

Monoclonal

Clone

Clone: ABAH-18

Tissue Specificity

Retinal pigment epithelium specific. .

Applications

WB, IP

Field of Research

Cancer, Cell Type Markers, Cofactors, Vitamins/Minerals, Metabolism, Neuroscience, Pathways and Processes, Sensory System, Signal Transduction, Tags & Cell Markers, Visual System, Vitamins/Minerals

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis- retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

60948 MW

Observed Molecular Weight

95 kDa

Gene Name Synonym

Retinoid isomerohydrolase

Subcellular Location

Cytoplasm . Cell membrane ; Lipid-anchor . Microsome membrane . Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated (By similarity) . Undergoes light-dependent intracellular transport to become more concentrated in the central region of the retina pigment epithelium cells. .

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.