Anti-BubR1 BUB1B Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-BubR1 BUB1B Rabbit Monoclonal Antibody catalog # M01564-1. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M01564-1

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta;2.7.11.1; MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L; Protein SSK1; BUB1B; BUBR1, MAD3L, SSK1

Gene Name

BUB1B

Gene ID

701

UniProt

O60566

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human BubR1

Clonality

Monoclonal

Clone

Clone: ABFG-2

Tissue Specificity

Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index. .

Applications

WB, IHC, IP, Flow Cytometry

Field of Research

Cancer, Cell Biology, Cell Cycle, Cell Division, Epigenetics and Nuclear Signaling, Kinases/Phosphatases, Spindle

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

119545 MW

Observed Molecular Weight

69 kDa

Gene Name Synonym

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Subcellular Location

Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and CASC5.

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.