Anti-Dnmt3b Rabbit Monoclonal Antibody Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-Dnmt3b Rabbit Monoclonal Antibody Rabbit Monoclonal Antibody catalog # M00319. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00319

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

DNA (cytosine-5) -methyltransferase 3B; Dnmt3b;2.1.1.37; DNA methyltransferase HsaIIIB; DNA MTase HsaIIIB; M.HsaIIIB; DNMT3B

Gene Name

DNMT3B

Gene ID

1789

UniProt

Q9UBC3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Dnmt3b

Clonality

Monoclonal

Clone

Clone: FO-4

Tissue Specificity

Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.

Applications

WB, ICC, IF

Field of Research

2339, Epigenetics and Nuclear Signaling

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co- repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity) . In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Function as transcriptional corepressor by associating with ZHX1. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

95751 MW

Observed Molecular Weight

84 kDa

Gene Name Synonym

DNA (cytosine-5) -methyltransferase 3B

Subcellular Location

Nucleus .

Protein Name

P2X purinoceptor 1

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.