- Catalog number
- M00896
- Size / count
- 100 µL/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Synonyms
- Protein lin-28 homolog B; Lin-28B; LIN28B; CSDD2
- Gene Name
- LIN28B
- Gene ID
- 389421
- UniProt
- Q6ZN17
- Host
- Rabbit
- Reactivity
- Human
- Immunogen
- A synthesized peptide derived from human Lin28B
- Clonality
- Monoclonal
- Clone
- Clone: EAA-12
- Tissue Specificity
- Expressed at high levels in the placenta and, at mucher lower, in testis and fetal liver (PubMed:16971064) . Isoform 1 is only detected in placenta and in moderately and poorly differentiated hepatocellular carcinoma cells (at protein level) . Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level) . Tends to be up-regulated in triple-negative (ER-, PR-, HER2-) breast tumors, as well as in liver, ovarian, and thyroid carcinomas (PubMed:22118463) . .
- Applications
- WB, ICC, IF, IP, Flow Cytometry
- Field of Research
- Cancer, Epigenetics and Nuclear Signaling, Tumor Biomarkers
- Purification
- Affinity-chromatography
- Concentration
- 0.5mg/ml
- Form
- Liquid
- Function
- Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed:22118463) . Does not act on pri-miR21 (PubMed:22118463) . The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed:19703396) . This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11- independent (PubMed:22118463) . Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity) . When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. .
- Storage Conditions
- Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 27084 MW
- Observed Molecular Weight
- 35 kDa
- Gene Name Synonym
- Protein lin-28 homolog B
- Subcellular Location
- Nucleus. Nucleus, nucleolus . Cytoplasm . Predominantly nucleolar (PubMed:22118463) . In Huh7 cells, predominantly cytoplasmic, with only a subset of cells exhibiting strong nuclear staining; however, the specificity of the polyclonal antibody used in these experiments has not been not documented (PubMed:16971064) . .
- Protein Name
- Glycogen synthase kinase-3 beta (gsk3b)
- Isotype
- Rabbit IgG
- Contents
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
