Anti-Raptor RPTOR Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-Raptor RPTOR Rabbit Monoclonal Antibody catalog # M01463. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M01463

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Regulatory-associated protein of mTOR; Raptor; p150 target of rapamycin (TOR) -scaffold protein; RPTOR; KIAA1303, RAPTOR

Gene Name

RPTOR

Gene ID

57521

UniProt

Q8N122

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Raptor

Clonality

Monoclonal

Clone

Clone: EGB-18

Tissue Specificity

Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Isoform 3 is widely expressed, with highest levels in nasal mucosa and pituitary and lowest in spleen. .

Applications

WB, IHC, ICC, IF, IP, Flow Cytometry

Field of Research

Adapters, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Cytoplasmic, Epigenetics and Nuclear Signaling, Signal Transduction

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E) . mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

149038 MW

Observed Molecular Weight

43 kDa

Gene Name Synonym

Regulatory-associated protein of mTOR

Subcellular Location

Cytoplasm. Lysosome. Cytoplasmic granule. Targeting to lysosomes depends on amino acid availability. In arsenite-stressed cells, accumulates in stress granules when associated with SPAG5 and association with lysosomes is drastically decreased.

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.