Anti-p95/NBS1 NBN Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-p95/NBS1 NBN Rabbit Monoclonal Antibody catalog # M00732. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00732

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Nibrin; Cell cycle regulatory protein p95; Nijmegen breakage syndrome protein 1; NBN; NBS, NBS1, P95

Gene Name

NBN

Gene ID

4683

UniProt

O60934

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human p95/NBS1

Clonality

Monoclonal

Clone

Clone: COF-14

Tissue Specificity

Ubiquitous. Expressed at high levels in testis.

Applications

WB, IHC, ICC, IF, IP, Flow Cytometry

Field of Research

Cancer, DNA/RNA, Epigenetics and Nuclear Signaling, Oncoproteins/Suppressors, Tumor Suppressors

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

84959 MW

Observed Molecular Weight

95 kDa

Gene Name Synonym

Nibrin

Subcellular Location

Nucleus . Nucleus, PML body . Chromosome, telomere . Localizes to discrete nuclear foci after treatment with genotoxic agents. .

Protein Name

T-cell surface glycoprotein CD4

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.