- Catalog number
- M00246
- Size / count
- 100 µL/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Synonyms
- Aurora kinase A;2.7.11.1; Aurora 2; Aurora/IPL1-related kinase 1; ARK-1; Aurora-related kinase 1; hARK1; Breast tumor-amplified kinase; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; AURKA; AIK, AIRK1, ARK1, AURA, AYK1, BTAK, IAK1, STK15, STK6
- Gene Name
- AURKA
- Gene ID
- 6790
- UniProt
- O14965
- Host
- Rabbit
- Reactivity
- Human, Mouse
- Immunogen
- A synthesized peptide derived from human Aurora A
- Clonality
- Monoclonal
- Clone
- Clone: DIH-1
- Tissue Specificity
- Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
- Applications
- WB, ICC, IF, Flow Cytometry
- Field of Research
- Cancer, Cell Biology, Cell Cycle, Cell Division, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction, Spindle, Tumor Biomarkers
- Purification
- Affinity-chromatography
- Concentration
- 0.5mg/ml
- Form
- Liquid
- Function
- Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis. .
- Storage Conditions
- Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 45809 MW
- Observed Molecular Weight
- 100-110 kDa
- Gene Name Synonym
- Aurora kinase A
- Subcellular Location
- Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cytoskeleton, cilium basal body . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Detected at the neurite hillock in developing neurons (By similarity) . Localizes at the centrosome in mitotic cells from early prophase until telophase, but also localizes to the spindle pole MTs from prophase to anaphase (PubMed:9606188, PubMed:17229885, PubMed:21225229) . Colocalized with SIRT2 at centrosome (PubMed:22014574) . Moves to the midbody during both telophase and cytokinesis (PubMed:17726514) . Associates with both the pericentriolar material (PCM) and centrioles (PubMed:22014574) . .
- Protein Name
- P2X purinoceptor 1
- Isotype
- Rabbit IgG
- Contents
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
