Anti-KDM1/LSD1 KDM1A Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-KDM1/LSD1 KDM1A Rabbit Monoclonal Antibody catalog # M00532-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00532-1

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Lysine-specific histone demethylase 1A;1.-.-.-; BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2; KDM1A; AOF2, KDM1, KIAA0601, LSD1

Gene Name

KDM1A

Gene ID

23028

UniProt

O60341

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human KDM1 / LSD1

Clonality

Monoclonal

Clone

Clone: DID-11

Tissue Specificity

Ubiquitously expressed. .

Applications

WB, IHC, ICC, IF, IP

Field of Research

Chromatin Modifying Enzymes, Epigenetics and Nuclear Signaling, Methylation

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR) -dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr- 6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E- cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

92903 MW

Observed Molecular Weight

100 kDa

Gene Name Synonym

Lysine-specific histone demethylase 1A

Subcellular Location

Nucleus .

Protein Name

T-cell surface glycoprotein CD4

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.