- Catalog number
- M02608
- Size / count
- 100 µL/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Synonyms
- Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8;2.7.11.24; JNK-46; Stress-activated protein kinase 1c; SAPK1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; MAPK8; JNK1, PRKM8, SAPK1, SAPK1C
- Gene Name
- MAPK8
- Gene ID
- 5599
- UniProt
- P45983/P53779
- Host
- Rabbit
- Reactivity
- Human, Mouse, Rat
- Immunogen
- A synthesized peptide derived from human JNK1/JNK3
- Clonality
- Monoclonal
- Clone
- Clone: ICC-13
- Tissue Specificity
- Expressed mainly in the nervous system, brain (fetus and adult) and hear (adult) . .
- Applications
- WB, IP
- Field of Research
- Cancer, Cancer Susceptibility, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, MAPK Pathway, Oncoproteins, Oncoproteins/Suppressors, Protein Phosphorylation, Proto-Oncogenes, Ser/Thr Kinases, Serine/Threonine Kinases, Signal Transduction, TLR Signaling, Transcription
- Purification
- Affinity-chromatography
- Concentration
- 0.5mg/ml
- Form
- Liquid
- Function
- Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692) . .
- Storage Conditions
- Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 48296 MW
- Observed Molecular Weight
- 290 kDa
- Gene Name Synonym
- Mitogen-activated protein kinase 8
- Subcellular Location
- Cytoplasm . Nucleus .
- Protein Name
- T-cell surface glycoprotein CD3 zeta chain
- Isotype
- Rabbit IgG
- Contents
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
