- Catalog number
- M01940
- Size / count
- 100 µL/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Synonyms
- Protein kinase C beta type; PKC-B; PKC-beta;2.7.11.13; PRKCB; PKCB, PRKCB1
- Gene Name
- PRKCB
- Gene ID
- 5579
- UniProt
- P05771
- Host
- Rabbit
- Reactivity
- Human, Mouse, Rat
- Immunogen
- A synthesized peptide derived from human PKC beta 2
- Clonality
- Monoclonal
- Clone
- Clone: DOC-16
- Tissue Specificity
- Widely expressed with highest levels in muscle, heart, placenta and spinal cord.
- Applications
- WB, IHC, ICC, IF, IP, Flow Cytometry
- Field of Research
- Domain Families, Epigenetics and Nuclear Signaling, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Transcription, Transcription Factors, Tyrosine Kinases, Zinc Finger
- Purification
- Affinity-chromatography
- Concentration
- 0.5mg/ml
- Form
- Liquid
- Function
- Calcium-activated, phospholipid- and diacylglycerol (DAG) -dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR) -dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1- MAPK/ERK signaling cascade. May participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity) . Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription. .
- Storage Conditions
- Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 76869 MW
- Observed Molecular Weight
- 28 kDa
- Gene Name Synonym
- Protein kinase C beta type
- Subcellular Location
- Cytoplasm . Nucleus . Membrane ; Peripheral membrane protein .
- Protein Name
- Lysosome-associated membrane glycoprotein 2 (Lamp2)
- Isotype
- Rabbit IgG
- Contents
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
