Anti-Caspase-14 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-Caspase-14 Rabbit Monoclonal Antibody catalog # M05143. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M05143

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Caspase-14; CASP-14;3.4.22.-; Caspase-14 subunit p17, mature form; Caspase-14 subunit p10, mature form; Caspase-14 subunit p20, intermediate form; Caspase-14 subunit p8, intermediate form; CASP14

Gene Name

CASP14

Gene ID

23581

UniProt

P31944

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Caspase-14

Clonality

Monoclonal

Clone

Clone: ICI-3

Tissue Specificity

Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level) . Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level) . In psoriatic skin only expressed at very low levels (PubMed:11175259) . The p17/10 mature form is expressed in epidermis stratum corneum, the p20/p8 intermediate form in epidermis upper granular cells of the stratum granulosum (PubMed:22825846) . .

Applications

WB, IHC, ICC, IF, IP, Flow Cytometry

Field of Research

Cell Biology, Intracellular

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum (PubMed:15556625) . Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin (By similarity) . In vitro has a preference for the substate [WY]-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF (PubMed:16854378, PubMed:19960512) . Involved in processing of prosaposin in the epidermis (By similarity) . May be involved in retinal pigment epithelium cell barrier function (PubMed:25121097) . Involved in DNA degradation in differentiated keratinocytes probably by cleaving DFFA/ICAD leading to liberation of DFFB/CAD (PubMed:24743736) . .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

27680 MW

Observed Molecular Weight

36 kDa

Gene Name Synonym

Caspase-14

Subcellular Location

Cytoplasm . Nucleus.

Protein Name

T-cell surface glycoprotein CD3 zeta chain

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.