Anti-HIF-2 alpha EPAS1 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-HIF-2 alpha EPAS1 Rabbit Monoclonal Antibody catalog # M01248. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M01248

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Endothelial PAS domain-containing protein 1; EPAS-1; Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73; bHLHe73; HIF-1-alpha-like factor; HLF; Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha; Member of PAS protein 2; PAS domain-containing protein 2; EPAS1; BHLHE73, HIF2A, MOP2, PASD2

Gene Name

EPAS1

Gene ID

2034

UniProt

Q99814

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human HIF-2 alpha

Clonality

Monoclonal

Clone

Clone: ADD-5

Tissue Specificity

Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.

Applications

WB, ICC, IF, Flow Cytometry

Field of Research

Cancer, Cancer Metabolism, Cardiovascular,2339, Domain Families, Epigenetics and Nuclear Signaling, Hlh/Leucine Zipper, Hypoxia, Invasion/Microenvironment, Metabolic Signaling Pathways, Metabolism, Metabolism Processes, Nucleotide Metabolism, Pathways and Processes, Response To Hypoxia, Transcription

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

96459 MW

Observed Molecular Weight

80 kDa

Gene Name Synonym

Endothelial PAS domain-containing protein 1

Subcellular Location

Nucleus . Nucleus speckle . Colocalizes with HIF3A in the nucleus and speckles. .

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/ml BSA.