Anti-KMT6 / EZH2 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-KMT6 / EZH2 Rabbit Monoclonal Antibody catalog # M00050-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00050-1

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Histone-lysine N-methyltransferase EZH2;2.1.1.43; ENX-1; Enhancer of zeste homolog 2; Lysine N-methyltransferase 6; EZH2; KMT6

Gene Name

EZH2

Gene ID

2146

UniProt

Q15910

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human KMT6 / EZH2

Clonality

Monoclonal

Clone

Clone: IDF-5

Tissue Specificity

Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis. .

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

Cancer,2339, Chromatin Modifying Enzymes, Chromatin Remodeling, Epigenetics and Nuclear Signaling, Methylation, Tumor Biomarkers

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys- 27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

85363 MW

Observed Molecular Weight

45 kDa

Gene Name Synonym

Histone-lysine N-methyltransferase EZH2

Subcellular Location

Nucleus .

Protein Name

P2X purinoceptor 1

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.