Anti-Apg3 (Atg3) Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-Apg3 (Atg3) Rabbit Monoclonal Antibody catalog # M01768. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M01768

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Ubiquitin-like-conjugating enzyme ATG3;6.3.2.-; Autophagy-related protein 3; APG3-like; hApg3; Protein PC3-96; ATG3; APG3, APG3L

Gene Name

ATG3

Gene ID

64422

UniProt

Q9NT62

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Apg3 (Atg3)

Clonality

Monoclonal

Clone

Clone: ABBC-1

Tissue Specificity

Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. .

Applications

WB, IHC, ICC, IF

Field of Research

DNA/Nucleotides, DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) . The ATG12- ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE) . This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt) . Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

35864 MW

Observed Molecular Weight

40 kDa

Gene Name Synonym

Ubiquitin-like-conjugating enzyme ATG3

Subcellular Location

Cytoplasm .

Protein Name

Lysosome-associated membrane glycoprotein 2 (Lamp2)

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.