Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody catalog # M00122-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00122-1

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Poly [ADP-ribose] polymerase 1; PARP-1;2.4.2.30; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD (+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1; PARP1; ADPRT, PPOL

Gene Name

PARP1

Gene ID

142

UniProt

P09874

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Cleaved PARP

Clonality

Monoclonal

Clone

Clone: HI-16

Tissue Specificity

Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. .

Applications

WB, IHC

Field of Research

Chromatin Modifying Enzymes, Epigenetics and Nuclear Signaling

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Involved in the base excision repair (BER) pathway, by catalyzing the poly (ADP-ribosyl) ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly (ADP- ribosyl) ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to ly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

113084 MW

Observed Molecular Weight

25 kDa

Gene Name Synonym

Poly [ADP-ribose] polymerase 1

Subcellular Location

Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.

Protein Name

P2X purinoceptor 1

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.