Anti-Histone H3.3 H3F3A Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-Histone H3.3 H3F3A Rabbit Monoclonal Antibody catalog # M06819. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M06819

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Histone H3.3; H3F3A; H3.3A, H3F3; PP781; H3F3B; H3.3B

Gene Name

H3F3A

Gene ID

3020

UniProt

P84243

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Histone H3.3

Clonality

Monoclonal

Clone

Clone: EBF-8

Tissue Specificity

Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level) . Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate. .

Applications

WB, IHC, ICC, IF

Field of Research

2339, Epigenetics and Nuclear Signaling, Histones

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

15328 MW

Observed Molecular Weight

94 kDa

Gene Name Synonym

Histone H3.3

Subcellular Location

Nucleus. Chromosome.

Protein Name

T-cell surface glycoprotein CD3 zeta chain

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.