Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody catalog # M30929. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Recombinant protein.

Specifications

Catalog number

M30929

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

CRISPR-associated endonuclease Cas9/Csn1 ;3.1.-.- ; SpCas9 ; SpyCas9 ; cas9 ; csn1 ; SPy_1046

Gene Name

Cas9

Gene ID

0

UniProt

Q99ZW2

Host

Rabbit

Reactivity

Recombinant protein

Immunogen

Recombinant fragment derived from Streptococcus pyogenes.

Clonality

Monoclonal

Clone

Clone: ABCI-3

Tissue Specificity

Widely expressed. .

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

Signaling Pathway

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids) (PubMed:21455174) . CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) . In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA; Cas9 only stabilizes the pre-crRNA:tracrRNA interaction and has no catalytic function in RNA processing (PubMed:24270795) . Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA) . The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed 3'-5' exonucleolytically. DNA-binding requires protein and both gRNAs, as does nuclease activity. Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. DNA strand separation and heteroduplex formation starts at PAM sites; PAM recognition is required for catalytic activity (PubMed:24476820) . Confers immunity against a plasmid with homology to the appropriate CRISPR spacer sequences (CRISPR interference) (PubMed:21455174) . .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

158441 MW

Observed Molecular Weight

50 kDa

Gene Name Synonym

CRISPR-associated endonuclease Cas9/Csn1

Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor.

Protein Name

T-cell surface glycoprotein CD3 zeta chain

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.