Anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal Antibody catalog # M00086. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00086

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Runt-related transcription factor 1; Acute myeloid leukemia 1 protein; Core-binding factor subunit alpha-2; CBF-alpha-2; Oncogene AML-1; Polyomavirus enhancer-binding protein 2 alpha B subunit; PEA2-alpha B; PEBP2-alpha B; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; RUNX1; AML1, CBFA2

Gene Name

RUNX1

Gene ID

861

UniProt

Q01196/Q13761/Q13950

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human RUNX1/RUNX2/RUNX3

Clonality

Monoclonal

Clone

Clone: DOG-18

Tissue Specificity

Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.

Applications

WB, IHC, ICC, IF, IP, Flow Cytometry

Field of Research

Neuroscience, Oncoproteins, Oncoproteins/Suppressors, Organogenesis, Skeletal Development, Stem Cells, Transcription, Transcription Factors

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B- dependent transcriptional activation. Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down- regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed:17377532) . .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

48737 MW

Observed Molecular Weight

75 kDa

Gene Name Synonym

Runt-related transcription factor 1

Subcellular Location

Nucleus.

Protein Name

P2X purinoceptor 1

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.