Anti-PI 3 Kinase p85 beta PIK3R2 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-PI 3 Kinase p85 beta PIK3R2 Rabbit Monoclonal Antibody catalog # M03363. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Rat.

Specifications

Catalog number

M03363

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Phosphatidylinositol 3-kinase regulatory subunit beta; PI3-kinase regulatory subunit beta; PI3K regulatory subunit beta; PtdIns-3-kinase regulatory subunit beta; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; PI3-kinase subunit p85-beta; PtdIns-3-kinase regulatory subunit p85-beta; PIK3R2

Gene Name

PIK3R2

Gene ID

5296

UniProt

O00459

Host

Rabbit

Reactivity

Human, Rat

Immunogen

A synthesized peptide derived from human PI 3 Kinase p85 beta

Clonality

Monoclonal

Clone

Clone: EOH-16

Tissue Specificity

Specifically expressed in immune and hematopoietic cells. Expressed in bone marrow and blood cells. Levels vary considerably within this compartment. Present in at least 74% of immature CD34+ cells, whereas within the more mature population of CD33+ cells, it is present in only 10% of cells. Present in the majority of T-cells, while it is present in a minority of B-cells (at protein level) . .

Applications

WB, IHC, ICC, IF, IP, Flow Cytometry

Field of Research

Immunology, Innate Immunity, Lipid Signaling, Signal Transduction, Signaling Pathway, TLR Signaling

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5- trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Inly regulates autophagy (PubMed:23604317) . Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin- dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (By similarity) . .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

81545 MW

Observed Molecular Weight

70 kDa

Gene Name Synonym

Phosphatidylinositol 3-kinase regulatory subunit beta

Subcellular Location

Cytoplasm . Cell membrane ; Peripheral membrane protein . Membrane raft . Cytoplasm, cytoskeleton . Membrane ; Peripheral membrane protein . Translocates to the plasma membrane when activated, translocation is probably due to different mechanisms depending on the stimulus and cell type. Translocates from the cytoplasm to membrane ruffles in a FCGR3/CD16-dependent manner. Colocalizes with FC-gamma-RIIB receptor (FCGR2B) or FCGR3/CD16 at membrane ruffles. Tyrosine phosphorylation may also participate in membrane localization. .

Protein Name

T-cell surface glycoprotein CD3 zeta chain

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.