Anti-JAK2 Monoclonal Antibody

About this product

Boster Bio Anti-JAK2 Monoclonal Antibody catalog # M00027. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00027

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Signal transducer and activator of transcription 3; Acute-phase response factor; STAT3; APRF

Gene Name

JAK2

Gene ID

3717

UniProt

O60674

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human JAK2 Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2.

Clonality

Monoclonal

Clone

Clone: COG-10

Tissue Specificity

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Applications

IP, IF, IHC, ICC, WB

Field of Research

Epigenetics and Nuclear Signaling, Signal Transduction, Cancer, Cell Biology, Oncoproteins/Suppressors, Nuclear Signaling Pathways, Oncoproteins, Cell Cycle, Cell Cycle Inhibitors, Kinases/Phosphatases, Protein Phosphorylation, Tyrosine Kinases

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6) - responsive elements identified in the promoters of various acute- phase protein genes. Activated by IL31 through IL31RA. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays an important role in host defense in methicillin-resistant S.aureus lung infection by regulating the expression of the antimicrobial lectin REG3G (By similarity) . .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

88068 MW

Observed Molecular Weight

131 kDa

Gene Name Synonym

Signal transducer and activator of transcription 3

Subcellular Location

Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.

Protein Name

P2X purinoceptor 1

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.