Anti-AKT1 Monoclonal Antibody

About this product

Boster Bio Anti-AKT1 Monoclonal Antibody catalog # M00024-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00024-1

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Signal transducer and activator of transcription 3; Acute-phase response factor; STAT3; APRF

Gene Name

AKT1

Gene ID

207

UniProt

P31749

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human AKT1 Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.

Clonality

Monoclonal

Clone

Clone: EBG-1

Tissue Specificity

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Applications

WB, IHC, ICC, IF, IP, Flow Cytometry

Field of Research

Apoptosis, Cancer, Cell Cycle, Cell Death, Epigenetics and Nuclear Signaling, Metabolism, Metabolism Processes, Nuclear, Pathways and Processes, Protein Phosphorylation, Ser/Thr Kinases, Serine/Threonine Kinases, Signal Transduction

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6) - responsive elements identified in the promoters of various acute- phase protein genes. Activated by IL31 through IL31RA. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays an important role in host defense in methicillin-resistant S.aureus lung infection by regulating the expression of the antimicrobial lectin REG3G (By similarity) . .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

88068 MW

Observed Molecular Weight

56 kDa

Gene Name Synonym

Signal transducer and activator of transcription 3

Subcellular Location

Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.

Protein Name

P2X purinoceptor 1

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.