Anti-HEC1 NDC80 Monoclonal Antibody

About this product

Boster Bio Anti-HEC1 NDC80 Monoclonal Antibody catalog # M01731. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M01731

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Eukaryotic translation initiation factor 5A-1; eIF-5A-1; eIF-5A1; Eukaryotic initiation factor 5A isoform 1; eIF-5A; Rev-binding factor; eIF-4D; EIF5A

Gene Name

NDC80

Gene ID

10403

UniProt

O14777

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human HEC1 Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.

Clonality

Monoclonal

Clone

Clone: ACBI-14

Tissue Specificity

Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level) . .

Applications

WB, IP

Field of Research

DNA/RNA, Epigenetics and Nuclear Signaling, RNA Processing, Translation

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

MRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF- alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

16832 MW

Observed Molecular Weight

68 kDa

Gene Name Synonym

Eukaryotic translation initiation factor 5A-1

Subcellular Location

Cytoplasm. Nucleus. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus, nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.

Protein Name

Lysosome-associated membrane glycoprotein 2 (Lamp2)

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.