Anti-Rad50 Monoclonal Antibody

About this product

Boster Bio Anti-Rad50 Monoclonal Antibody catalog # M00347. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00347

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Ubiquitin-like modifier-activating enzyme ATG7; ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7; APG7-like; hAGP7; Ubiquitin-activating enzyme E1-like protein; ATG7; APG7L

Gene Name

RAD50

Gene ID

10111

UniProt

Q92878

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Rad50 Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A.

Clonality

Monoclonal

Clone

Clone: ACCG-18

Tissue Specificity

Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. .

Applications

WB, ICC, IF

Field of Research

Chromosome Structure, DNA/RNA, DNA Damage & Repair, Epigenetics and Nuclear Signaling

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

E1-like activating enzyme involved in the 2 ubiquitin- like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

77960 MW

Observed Molecular Weight

35 kDa

Gene Name Synonym

Ubiquitin-like modifier-activating enzyme ATG7

Subcellular Location

Cytoplasm . Preautophagosomal structure . Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme. .

Protein Name

T-cell surface glycoprotein CD4

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.