Anti-DNA Ligase IV Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-DNA Ligase IV Rabbit Monoclonal Antibody catalog # M01685. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.

Specifications

Catalog number

M01685

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; CD146; MCAM; MUC18

Gene Name

LIG4

Gene ID

3981

UniProt

P49917

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human DNA Ligase IV

Clonality

Monoclonal

Clone

Clone: ADHG-12

Tissue Specificity

Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.

Applications

WB, IHC, ICC, IF

Field of Research

Cancer, Cell Adhesion, Cell Adhesion Molecules, Cell Type Markers, Cytoskeleton/ECM, Immunology, Invasion/Microenvironment, Signal Transduction, Stem Cells

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

71607 MW

Observed Molecular Weight

120 kDa

Gene Name Synonym

Cell surface glycoprotein MUC18

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Name

Lysosome-associated membrane glycoprotein 2 (Lamp2)

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.