Anti-RING2 / RING1B / RNF2 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-RING2 / RING1B / RNF2 Rabbit Monoclonal Antibody catalog # M01209. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M01209

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Vinculin; Metavinculin; MV; VCL

Gene Name

RNF2

Gene ID

6045

UniProt

Q99496

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human RING2 / RING1B / RNF2 E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals.

Clonality

Monoclonal

Clone

Clone: AEDC-18

Tissue Specificity

Metavinculin is muscle-specific.

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

2339, Chromatin Remodeling, Epigenetics and Nuclear Signaling

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Actin filament (F-actin) -binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

123799 MW

Observed Molecular Weight

17 kDa

Gene Name Synonym

Vinculin

Subcellular Location

Cytoplasm, cytoskeleton . Cell junction, adherens junction . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, focal adhesion . Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II- dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions (By similarity) . Colocalizes with LIMD1 in the focal adhesions. .

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.