Anti-PTBP1 Monoclonal Antibody

About this product

Boster Bio Anti-PTBP1 Monoclonal Antibody catalog # M01798. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M01798

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Heterogeneous nuclear ribonucleoprotein K; hnRNP K; Transformation up-regulated nuclear protein; TUNP; HNRNPK; HNRPK

Gene Name

PTBP1

Gene ID

5725

UniProt

P26599

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human PTBP1

Clonality

Monoclonal

Clone

Clone: AFEO-16

Tissue Specificity

Expressed in brain and lymphoblast.

Applications

WB, IHC, ICC, IF

Field of Research

Chromatin Binding Proteins, DNA/RNA, DNA/RNA Binding, Epigenetics and Nuclear Signaling, RNA Processing, Splicing

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

One of the major pre-mRNA-binding proteins. Binds tenaciously to poly (C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly (C) single- stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity) . As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

50976 MW

Observed Molecular Weight

40 kDa

Gene Name Synonym

Heterogeneous nuclear ribonucleoprotein K

Subcellular Location

Cytoplasm . Nucleus, nucleoplasm . Cell projection, podosome . Recruited to p53/TP53- responsive promoters, in the presence of functional p53/TP53 (PubMed:16360036) . In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear (PubMed:18775702) .

Protein Name

Lysosome-associated membrane glycoprotein 2 (Lamp2)

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.