- Catalog number
- M00359-3
- Size / count
- 100 µL/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Synonyms
- Cyclin-dependent kinase 6;2.7.11.22; Cell division protein kinase 6; Serine/threonine-protein kinase PLSTIRE; CDK6; CDKN6
- Gene Name
- TG
- Gene ID
- 7038
- UniProt
- P01266
- Host
- Rabbit
- Reactivity
- Human, Mouse, Rat
- Immunogen
- A synthesized peptide derived from human Thyroglobulin Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3) .
- Clonality
- Monoclonal
- Clone
- Clone: AFEC-20
- Tissue Specificity
- Expressed ubiquitously. Accumulates in squamous cell carcinomas, proliferating hematopoietic progenitor cells, beta-cells of pancreatic islets of Langerhans, and neuroblastomas. Reduced levels in differentiating cells. .
- Applications
- WB, IHC, ICC, IF, Flow Cytometry
- Field of Research
- Cancer, Cell Biology, Cell Cycle, Epigenetics and Nuclear Signaling, Kinases/Phosphatases
- Purification
- Affinity-chromatography
- Concentration
- 0.5mg/ml
- Form
- Liquid
- Function
- Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells) . Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases (PubMed:23918663) . .
- Storage Conditions
- Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 36938 MW
- Observed Molecular Weight
- 37 kDa
- Gene Name Synonym
- Cyclin-dependent kinase 6
- Subcellular Location
- Cytoplasm. Nucleus. Cell projection, ruffle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Localized to the ruffling edge of spreading fibroblasts. Kinase activity only in nucleus. Localized to the cytosol of neurons and showed prominent staining around either side of the nucleus (By similarity) . Present in the cytosol and in the nucleus in interphase cells and at the centrosome during mitosis from prophase to telophase (PubMed:23918663) . .
- Protein Name
- T-cell surface glycoprotein CD4
- Isotype
- Rabbit IgG
- Contents
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
