Anti-Nck NCK1 Monoclonal Antibody

About this product

Boster Bio Anti-Nck NCK1 Monoclonal Antibody catalog # M02260. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M02260

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Oligodendrocyte transcription factor 2; Oligo2; Class B basic helix-loop-helix protein 1; bHLHb1; Class E basic helix-loop-helix protein 19; bHLHe19; Protein kinase C-binding protein 2; Protein kinase C-binding protein RACK17; OLIG2; BHLHB1, BHLHE19, PRKCBP2, RACK17

Gene Name

NCK1

Gene ID

4690

UniProt

P16333

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Nck Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1.

Clonality

Monoclonal

Clone

Clone: AFEG-14

Tissue Specificity

Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable. .

Applications

WB, IHC, ICC, IF, IP, Flow Cytometry

Field of Research

Actin Assembly, Actin, etc., Adapters, Cancer, Cancer Susceptibility, Cytoplasmic, Cytoskeleton, Cytoskeleton/ECM, Epigenetics and Nuclear Signaling, Microfilaments, Oncoproteins, Oncoproteins/Suppressors, Proto-Oncogenes, Signal Transduction, Transcription

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development (By similarity) . .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

32385 MW

Observed Molecular Weight

16 kDa

Gene Name Synonym

Oligodendrocyte transcription factor 2

Subcellular Location

Nucleus . Cytoplasm . The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2. .

Protein Name

Lysosome-associated membrane glycoprotein 2 (Lamp2)

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.