Anti-TDT DNTT Monoclonal Antibody

About this product

Boster Bio Anti-TDT DNTT Monoclonal Antibody catalog # M05546. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M05546

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Drebrin; Developmentally-regulated brain protein; DBN1; D0S117E

Gene Name

DNTT

Gene ID

1791

UniProt

P04053

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human TDT Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.

Clonality

Monoclonal

Clone

Clone: AFHA-4

Tissue Specificity

Brain neurons. Also found in the heart, placenta, skeletal muscle, kidney and pancreas. Expressed in peripheral blood lymphocytes, including T-cells (at protein level) . .

Applications

WB, IHC

Field of Research

DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Drebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites. Required for actin polymerization at immunological synapses (IS) and for CXCR4 recruitment to IS. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

71429 MW

Observed Molecular Weight

10 kDa

Gene Name Synonym

Drebrin

Subcellular Location

Cytoplasm . Cytoplasm, cell cortex . Cell junction . Cell projection . Cell projection, growth cone . In the absence of antigen, evenly distributed throughout subcortical regions of the T-cell membrane and cytoplasm. In the presence of antigen, distributes to the immunological synapse forming at the T-cell-APC contact area, where it localizes at the peripheral and distal supramolecular activation clusters (SMAC) (PubMed:20215400) . Colocalized with DBN1, RUFY3 and F-actin at the transitional domain of the axonal growth cone (By similarity) . .

Protein Name

T-cell surface glycoprotein CD3 zeta chain

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.