Anti-Apg12 (Atg12) Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-Apg12 (Atg12) Rabbit Monoclonal Antibody catalog # M00820-2. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Specifications

Catalog number

M00820-2

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Ubiquitin-like protein ATG12; Autophagy-related protein 12; APG12-like; ATG12; APG12, APG12L

Gene Name

ATG12

Gene ID

9140

UniProt

O94817

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Apg12 (Atg12)

Clonality

Monoclonal

Clone

Clone: 17A34

Tissue Specificity

Ubiquitous. .

Applications

WB, IHC, ICC, IF, IP, Flow Cytometry

Field of Research

Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction, Cancer, Cell Biology, Nucleotide Metabolism

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

15113 MW

Observed Molecular Weight

95 kDa

Gene Name Synonym

Ubiquitin-like protein ATG12

Subcellular Location

Cytoplasm . Preautophagosomal structure membrane ; Peripheral membrane protein . TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane.

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)

Isotype

IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.