- Catalog number
- M00707
- Size / count
- 100 µL/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Synonyms
- High affinity nerve growth factor receptor;2.7.10.1; Neurotrophic tyrosine kinase receptor type 1; TRK1-transforming tyrosine kinase protein; Tropomyosin-related kinase A; Tyrosine kinase receptor; Tyrosine kinase receptor A; Trk-A; gp140trk; p140-TrkA; NTRK1; MTC, TRK, TRKA
- Gene Name
- RNF8
- Gene ID
- 9025
- UniProt
- O76064
- Host
- Rabbit
- Reactivity
- Human, Rat
- Immunogen
- A synthesized peptide derived from human RNF8
- Clonality
- Monoclonal
- Clone
- Clone: 18R02
- Tissue Specificity
- Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.
- Applications
- WB
- Field of Research
- Cancer, Growth and Development, Neurology Process, Neuroscience, Oncoproteins, Oncoproteins/Suppressors
- Purification
- Affinity-chromatography
- Concentration
- 0.5mg/ml
- Form
- Liquid
- Function
- Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand, it can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras- PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.
- Storage Conditions
- Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 87497 MW
- Observed Molecular Weight
- 28 kDa
- Gene Name Synonym
- High affinity nerve growth factor receptor
- Subcellular Location
- Cell membrane ; Single-pass type I membrane protein . Early endosome membrane ; Single-pass type I membrane protein . Late endosome membrane ; Single-pass type I membrane protein . Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity) . .
- Protein Name
- T-cell surface glycoprotein CD4
- Isotype
- IgG
- Contents
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
