- Catalog number
- M02709
- Size / count
- 100 µL/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Synonyms
- Mitogen-activated protein kinase 9; MAP kinase 9; MAPK 9;2.7.11.24; JNK-55; Stress-activated protein kinase 1a; SAPK1a; Stress-activated protein kinase JNK2; c-Jun N-terminal kinase 2; MAPK9; JNK2, PRKM9, SAPK1A
- Gene Name
- NKX3-1
- Gene ID
- 4824
- UniProt
- Q99801
- Host
- Rabbit
- Reactivity
- Human
- Immunogen
- A synthesized peptide derived from human Nkx3.1
- Clonality
- Monoclonal
- Clone
- Clone: 19N21
- Tissue Specificity
- Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis. .
- Applications
- WB, IHC
- Field of Research
- Cancer, Cell Biology, Immunology, Innate Immunity, MAPK Pathway, Metabolism, Oncoproteins, Oncoproteins/Suppressors, Oxidative Stress, Pathways and Processes, Protein Phosphorylation, Redox Metabolism, Ser/Thr Kinases, Serine/Threonine Kinases, Signal Transduction, TLR Signaling, Tumor Biomarkers
- Purification
- Affinity-chromatography
- Concentration
- 0.5mg/ml
- Form
- Liquid
- Function
- Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692) . .
- Storage Conditions
- Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 48139 MW
- Observed Molecular Weight
- 35 kDa
- Gene Name Synonym
- Mitogen-activated protein kinase 9
- Subcellular Location
- Cytoplasm . Nucleus .
- Protein Name
- T-cell surface glycoprotein CD3 zeta chain
- Isotype
- IgG
- Contents
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
