Anti-PIAS1 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-PIAS1 Rabbit Monoclonal Antibody catalog # M01707-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M01707-1

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

E3 SUMO-protein ligase PIAS1;6.3.2.-; DEAD/H box-binding protein 1; Gu-binding protein; GBP; Protein inhibitor of activated STAT protein 1; RNA helicase II-binding protein; PIAS1; DDXBP1

Gene Name

PIAS1

Gene ID

8554

UniProt

O75925

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human PIAS1

Clonality

Monoclonal

Clone

Clone: 19P34

Tissue Specificity

Expressed in numerous tissues with highest level in testis. .

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

2339, Co-Activators/Co-Repressors, Epigenetics and Nuclear Signaling, Nuclear Receptors, Nuclear Signaling Pathways, Transcription, Ubiquitin & Ubiquitin Like Modifiers

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity) . Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (By similarity) . .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

71836 MW

Observed Molecular Weight

72 kDa

Gene Name Synonym

E3 SUMO-protein ligase PIAS1

Subcellular Location

Nucleus speckle . Nucleus, PML body . Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.

Protein Name

Lysosome-associated membrane glycoprotein 2 (Lamp2)

Isotype

IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.