Anti-RENT1 / hUPF1 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-RENT1 / hUPF1 Rabbit Monoclonal Antibody catalog # M00900-2. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00900-2

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Protein lin-28 homolog B; Lin-28B; LIN28B; CSDD2

Gene Name

UPF1

Gene ID

5976

UniProt

Q92900

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human RENT1 / hUPF1

Clonality

Monoclonal

Clone

Clone: 19U80

Tissue Specificity

Expressed at high levels in the placenta and, at mucher lower, in testis and fetal liver (PubMed:16971064) . Isoform 1 is only detected in placenta and in moderately and poorly differentiated hepatocellular carcinoma cells (at protein level) . Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level) . Tends to be up-regulated in triple-negative (ER-, PR-, HER2-) breast tumors, as well as in liver, ovarian, and thyroid carcinomas (PubMed:22118463) . .

Applications

WB, IHC, ICC, IF, IP, Flow Cytometry

Field of Research

Cancer, Epigenetics and Nuclear Signaling, Tumor Biomarkers

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed:22118463) . Does not act on pri-miR21 (PubMed:22118463) . The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed:19703396) . This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11- independent (PubMed:22118463) . Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity) . When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

124 kDa

Observed Molecular Weight

130 kDa

Gene Name Synonym

Protein lin-28 homolog B

Subcellular Location

Nucleus. Nucleus, nucleolus . Cytoplasm . Predominantly nucleolar (PubMed:22118463) . In Huh7 cells, predominantly cytoplasmic, with only a subset of cells exhibiting strong nuclear staining; however, the specificity of the polyclonal antibody used in these experiments has not been not documented (PubMed:16971064) . .

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)

Isotype

IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.