Anti-Lamin A/C Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-Lamin A/C Rabbit Monoclonal Antibody catalog # M00438-5. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00438-5

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Prelamin-A/C; Lamin-A/C;70 kDa lamin; Renal carcinoma antigen NY-REN-32; LMNA; LMN1

Gene Name

LMNA

Gene ID

4000

UniProt

P02545

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Lamin A/C

Clonality

Monoclonal

Clone

Clone: 22L53

Tissue Specificity

In the arteries, prelamin-A/C accumulation is not observed in young healthy vessels but is prevalent in medial vascular smooth muscle cells (VSMCs) from aged individuals and in atherosclerotic lesions, where it often colocalizes with senescent and degenerate VSMCs. Prelamin-A/C expression increases with age and disease. In normal aging, the accumulation of prelamin-A/C is caused in part by the down-regulation of ZMPSTE24/FACE1 in response to oxidative stress. .

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

Cytoskeleton, Cytoskeleton/ECM, Intermediate Filaments, Nucleus, Signal Transduction, Subcellular Markers, Tags & Cell Markers

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation. Required for osteoblastogenesis and bone formation. Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

74139 MW

Observed Molecular Weight

70-74 kDa

Gene Name Synonym

Prelamin-A/C

Subcellular Location

Nucleus. Nucleus envelope. Nucleus lamina. Nucleus, nucleoplasm. Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleaveage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature lamin- A/C, which can then be inserted into the nuclear lamina. EMD is required for proper localization of non-farnesylated prelamin-A/C.

Protein Name

T-cell surface glycoprotein CD4

Isotype

IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.