Anti-CRKII Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-CRKII Rabbit Monoclonal Antibody catalog # M02533-3. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M02533-3

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Proline-, glutamic acid- and leucine-rich protein 1; Modulator of non-genomic activity of estrogen receptor; Transcription factor HMX3; PELP1; HMX3, MNAR

Gene Name

CRK

Gene ID

1398

UniProt

P46108

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human CRKII

Clonality

Monoclonal

Clone

Clone: 24C53

Tissue Specificity

Widely expressed. .

Applications

WB, ICC, IF, IP, Flow Cytometry

Field of Research

Co-Activators/Co-Repressors, Epigenetics and Nuclear Signaling, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence- specific transcription factors. Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner. Can facilitate ER non-genomic signaling via SRC and PI3K interaction in the cytosol. Plays a role in E2-mediated cell cycle progression by interacting with RB1. May have important functional implications in ER/growth factor cross-talk. Interacts with several growth factor signaling components including EGFR and HRS. Involved in nuclear receptor signaling via its interaction with AR and NR3C1. May promote tumorigenesis via its interaction with and modulation of several oncogenes including SRC, PI3K, STAT3 and EGFR. Plays a role in cancer cell metastasis via its ability to modulate E2-mediated cytoskeleton changes and cell migration via its interaction with SRC and PI3K. Functions as the key stabilizing component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

119700 MW

Observed Molecular Weight

38 kDa

Gene Name Synonym

Proline-, glutamic acid- and leucine-rich protein 1

Subcellular Location

Nucleus, nucleoplasm . Nucleus. Cytoplasm. Mainly found in the nucleoplasm, with low levels detected in the cytoplasm (By similarity) . Also found associated with the plasma membrane. Mainly in cytoplasm in a subset of breast tumors. Localization is widely deregulated in endometrial cancers with predominantly cytoplasm localization in high-grade endometrial tumors. .

Protein Name

T-cell surface glycoprotein CD3 zeta chain

Isotype

IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.