Anti-DOK1 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-DOK1 Rabbit Monoclonal Antibody catalog # M03039. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M03039

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Ubiquitin-conjugating enzyme E2 R1;6.3.2.19; Ubiquitin-conjugating enzyme E2-32 kDa complementing; Ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1; CDC34; UBCH3, UBE2R1

Gene Name

DOK1

Gene ID

1796

UniProt

Q99704

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human DOK1

Clonality

Monoclonal

Clone

Clone: 25D44

Tissue Specificity

Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription. .

Applications

WB, ICC, IF

Field of Research

Chromatin Modifying Enzymes, Epigenetics and Nuclear Signaling, Ubiquitin & Ubiquitin Like Modifiers

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF (FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF (SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5- mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

26737 MW

Observed Molecular Weight

65 kDa

Gene Name Synonym

Ubiquitin-conjugating enzyme E2 R1

Subcellular Location

Cytoplasm. Nucleus. The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase.

Protein Name

T-cell surface glycoprotein CD3 zeta chain

Isotype

IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.