Anti-Phospho-Nucleolin (T76) Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-Phospho-Nucleolin (T76) Rabbit Monoclonal Antibody catalog # P00228-1. Tested in WB, IHC applications. This antibody reacts with Human.

Specifications

Catalog number

P00228-1

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Phospho Site

T76

Synonyms

Leucine-rich repeat serine/threonine-protein kinase 2;2.7.11.1; Dardarin; LRRK2; PARK8

Gene Name

NCL

Gene ID

4691

UniProt

P19338

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Phospho-Nucleolin (T76)

Clonality

Monoclonal

Clone

Clone: 29N32

Tissue Specificity

Expressed in the brain. Expressed in pyramidal neurons in all cortical laminae of the visual cortex, in neurons of the substantia nigra pars compacta and caudate putamen (at protein level) . Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas. .

Applications

WB, IHC

Field of Research

Neurodegenerative Disease, Neurology Process, Neuroscience

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Positively regulates autophagy through a calcium- dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS) . Plays a role in synaptic vesicle trafficking. Phosphorylates PRDX3. Has GTPase activity. May play a role in the phosphorylation of proteins central to Parkinson disease. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

286103 MW

Observed Molecular Weight

100-110 kDa

Gene Name Synonym

Leucine-rich repeat serine/threonine-protein kinase 2

Subcellular Location

Membrane; Peripheral membrane protein. Cytoplasm. Perikaryon. Mitochondrion. Golgi apparatus. Cell projection, axon. Cell projection, dendrite. Endoplasmic reticulum . Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Endosome . Lysosome . Mitochondrion outer membrane . Mitochondrion inner membrane . Mitochondrion matrix . Predominantly associated with intracytoplasmic vesicular and membranous structures (By similarity) . Localized in the cytoplasm and associated with cellular membrane structures. Predominantly associated with the mitochondrial outer membrane of the mitochondria. Colocalized with RAB29 along tubular structures emerging from Golgi apparatus. Localizes in intracytoplasmic punctate structures of neuronal perikarya and dendritic and axonal processes. .

Isotype

IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.