Anti-Phospho-MDM2 (S166) Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-Phospho-MDM2 (S166) Rabbit Monoclonal Antibody catalog # P00054-2. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

P00054-2

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Phospho Site

S166

Synonyms

Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110; RB1

Gene Name

MDM2

Gene ID

4193

UniProt

Q00987

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Phospho-MDM2 (S166)

Clonality

Monoclonal

Clone

Clone: 29M35

Tissue Specificity

Expressed in the retina.

Applications

WB, IHC

Field of Research

Cancer, Cancer Susceptibility, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Epigenetics and Nuclear Signaling, Nucleus, Oncoproteins/Suppressors, Subcellular Markers, Tags & Cell Markers, Transcription, Tumor Suppressors

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. ly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity) . In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

106159 MW

Observed Molecular Weight

90-100 kDa

Gene Name Synonym

Retinoblastoma-associated protein

Subcellular Location

Nucleus .

Isotype

IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.