Anti-SLC12A1 / NKCC2 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-SLC12A1 / NKCC2 Rabbit Monoclonal Antibody catalog # M04473. Tested in WB application. This antibody reacts with Human.

Specifications

Catalog number

M04473

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

5'-AMP-activated protein kinase subunit gamma-1; AMPK gamma1; AMPK subunit gamma-1; AMPKg; PRKAG1

Gene Name

SLC12A1

Gene ID

6557

UniProt

Q13621

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human SLC12A1 / NKCC2

Clonality

Monoclonal

Clone

Clone: 29S41

Tissue Specificity

Leukocytes.

Applications

WB

Field of Research

Cell Adhesion, Cell Type Markers, Cytoskeleton/ECM, Hematopoietic Progenitors, Immunology, Integrins, Myeloid, Signal Transduction, Stem Cells

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by inly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

37579 MW

Observed Molecular Weight

150 kDa

Gene Name Synonym

5'-AMP-activated protein kinase subunit gamma-1

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Name

T-cell surface glycoprotein CD3 zeta chain

Isotype

IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.