Anti-SAFB Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-SAFB Rabbit Monoclonal Antibody catalog # M03458-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M03458-1

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

E3 ubiquitin-protein ligase BRE1A; BRE1-A; hBRE1;6.3.2.-; RING finger protein 20; RNF20; BRE1A

Gene Name

SAFB

Gene ID

6294

UniProt

Q15424

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human SAFB

Clonality

Monoclonal

Clone

Clone: 30S09

Tissue Specificity

Expressed in the normal brain and also in malignant gliomas (at protein level) . .

Applications

WB, IHC, ICC, IF

Field of Research

Cell Biology, Chromatin Modifying Enzymes, Epigenetics and Nuclear Signaling, Histones, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ring Finger E3 Ligase, Ubiquitin E3 Enzymes

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1) . H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively) . It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

113662 MW

Observed Molecular Weight

150 kDa

Gene Name Synonym

E3 ubiquitin-protein ligase BRE1A

Subcellular Location

Nucleus .

Protein Name

T-cell surface glycoprotein CD3 zeta chain

Isotype

IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.