Anti-Phospho-p130 (T986) Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-Phospho-p130 (T986) Rabbit Monoclonal Antibody catalog # P02118. Tested in WB, IP applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number

P02118

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Phospho Site

T986

Synonyms

Replication protein A 32 kDa subunit; RP-A p32; Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit; RP-A p34; RPA2; REPA2, RPA32, RPA34

Gene Name

RBL2

Gene ID

5934

UniProt

Q08999

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human Phospho-p130 (T986)

Clonality

Monoclonal

Clone

Clone: 30R10

Tissue Specificity

Expressed in brain, and to a lesser extent in lung, kidney and testis.

Applications

WB, IP

Field of Research

DNA/RNA, DNA Damage & Repair, DNA Damage Response, DNA Synthesis, Epigenetics and Nuclear Signaling

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

29247 MW

Observed Molecular Weight

128 kDa

Gene Name Synonym

Replication protein A 32 kDa subunit

Subcellular Location

Nucleus . Nucleus, PML body . Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner. .

Isotype

IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.