- Catalog number
- EZ0954
- Size / count
- 5 Plates/Kit
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Epidermal growth factor (EGF) is a growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation by binding to its receptor EGFR. EGF locus is mapped to 4q21-4qter. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bones. EGF results in cellular proliferation, differentiation, and survival while plays a physiological role in the maintenance of oro-esophageal and gastric tissue integrity.
- Synonyms
- Pro-epidermal growth factor; EGF; Epidermal growth factor; Egf
- Gene Name
- EGF
- Gene ID
- 25313
- UniProt
- P07522
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: N974-R1026
- Applications
- ELISA
- Field of Research
- Growth Factors/Hormones, Signal Transduction
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
- Detection Range
- 7.8 pg/mL - 500 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 90 232 10 96 211 Standard deviation 0.75 5.31 16.24 0.83 6.91 16.66 CV (%) 6.9% 5.9% 7% 8.3% 7.2% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 90 232 10 96 211 Standard deviation 0.75 5.31 16.24 0.83 6.91 16.66 CV (%) 6.9% 5.9% 7% 8.3% 7.2% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 11 90 232 10 96 211 Mean (pg/ml) 11 90 232 10 96 211 Standard deviation 0.75 5.31 16.24 0.83 6.91 16.66 Standard deviation 0.75 5.31 16.24 0.83 6.91 16.66 CV (%) 6.9% 5.9% 7% 8.3% 7.2% 7.9% CV (%) 6.9% 5.9% 7% 8.3% 7.2% 7.9%
- Function
- EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity) .
- Components
- Catalog number Description Quantity Storage of opened/reconstituted material EZ0954-CA Rabbit anti- rat Egf polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0954-DA Biotinylated goat anti- rat Egf polyclonal antibody (Detection Antibody) 500 μl, 2.5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0954-ST Lyophilized recombinant rat Egf standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0954-CA Rabbit anti- rat Egf polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0954-DA Biotinylated goat anti- rat Egf polyclonal antibody (Detection Antibody) 500 μl, 2.5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0954-ST Lyophilized recombinant rat Egf standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0954-CA Rabbit anti- rat Egf polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0954-CA Rabbit anti- rat Egf polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0954-DA Biotinylated goat anti- rat Egf polyclonal antibody (Detection Antibody) 500 μl, 2.5 μg/mL (recommended dilution 1:100) EZ0954-DA Biotinylated goat anti- rat Egf polyclonal antibody (Detection Antibody) 500 μl, 2.5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0954-ST Lyophilized recombinant rat Egf standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. EK0954-ST Lyophilized recombinant rat Egf standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit.
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant rat EGF
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Epidermal growth factor
- Subcellular Location
- Membrane, Single-pass type I membrane protein
- Sample Data
- Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.033 0.129 0.207 0.406 0.715 1.21 2.012 2.55 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.033 0.129 0.207 0.406 0.715 1.21 2.012 2.55 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.033 0.129 0.207 0.406 0.715 1.21 2.012 2.55 O.D. 0.033 0.129 0.207 0.406 0.715 1.21 2.012 2.55
- Material Required But Not Provided
- 1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
