- Catalog number
- EZ0384
- Size / count
- 5 Plates/Kit
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Insulin-like growth factor-binding protein 2 (IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment.
- Synonyms
- Insulin-like growth factor-binding protein 2; IBP-2; IGF-binding protein 2; IGFBP-2; IGFBP2; BP2, IBP2
- Gene Name
- IGFBP2
- Gene ID
- 3485
- UniProt
- P18065
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E40-Q328
- Applications
- ELISA
- Field of Research
- Cancer, Extracellular Matrix, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Signal Transduction
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 128 598 1114 133 553 1221 Standard deviation 5.88 47.24 61.27 7.84 54.19 83.02 CV (%) 4.6% 7.9% 5.5% 5.9% 9.8% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 128 598 1114 133 553 1221 Standard deviation 5.88 47.24 61.27 7.84 54.19 83.02 CV (%) 4.6% 7.9% 5.5% 5.9% 9.8% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 128 598 1114 133 553 1221 Mean (pg/ml) 128 598 1114 133 553 1221 Standard deviation 5.88 47.24 61.27 7.84 54.19 83.02 Standard deviation 5.88 47.24 61.27 7.84 54.19 83.02 CV (%) 4.6% 7.9% 5.5% 5.9% 9.8% 6.8% CV (%) 4.6% 7.9% 5.5% 5.9% 9.8% 6.8%
- Function
- Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
- Components
- Catalog number Description Quantity Storage of opened/reconstituted material EZ0384-CA Rabbit anti- human IGFBP2 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0384-DA Biotinylated goat anti- human IGFBP2 polyclonal antibody (Detection Antibody) 500 μl, 2.5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0384-ST Lyophilized recombinant human IGFBP2 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0384-CA Rabbit anti- human IGFBP2 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0384-DA Biotinylated goat anti- human IGFBP2 polyclonal antibody (Detection Antibody) 500 μl, 2.5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0384-ST Lyophilized recombinant human IGFBP2 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0384-CA Rabbit anti- human IGFBP2 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0384-CA Rabbit anti- human IGFBP2 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0384-DA Biotinylated goat anti- human IGFBP2 polyclonal antibody (Detection Antibody) 500 μl, 2.5 μg/mL (recommended dilution 1:100) EZ0384-DA Biotinylated goat anti- human IGFBP2 polyclonal antibody (Detection Antibody) 500 μl, 2.5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0384-ST Lyophilized recombinant human IGFBP2 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. EK0384-ST Lyophilized recombinant human IGFBP2 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit.
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human IGFBP2
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Insulin-like growth factor binding protein 2, 36kDa
- Subcellular Location
- Secreted
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.137 0.218 0.283 0.422 0.691 1.201 1.916 2.423 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.137 0.218 0.283 0.422 0.691 1.201 1.916 2.423 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.137 0.218 0.283 0.422 0.691 1.201 1.916 2.423 O.D. 0.137 0.218 0.283 0.422 0.691 1.201 1.916 2.423
- Material Required But Not Provided
- 1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
