- Catalog number
- EZ0707
- Size / count
- 5 Plates/Kit
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain. The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.
- Synonyms
- T-lymphocyte activation antigen CD80; Activation B7-1 antigen; BB1; CTLA-4 counter-receptor B7.1; B7; CD80; CD80; CD28LG, CD28LG1, LAB7
- Gene Name
- CD80
- Gene ID
- 941
- UniProt
- P33681
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: V35-N242
- Tissue Specificity
- Expressed on activated B-cells, macrophages and dendritic cells.
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, B Cells, Hematopoietic Progenitors, Immunology, Lymphoid, Myeloid, Stem Cells
- Sample Type
- Cell culture supernatants and serum.
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Function
- Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. .
- Components
- Catalog number Description Quantity Storage of opened/reconstituted material EZ0707-CA Mouse anti-human B7-1/CD80 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0707-DA Biotinylated goat anti-human B7-1/CD80 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ0707-ST Lyophilized recombinant human B7-1/CD80 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0707-CA Mouse anti-human B7-1/CD80 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0707-DA Biotinylated goat anti-human B7-1/CD80 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ0707-ST Lyophilized recombinant human B7-1/CD80 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0707-CA Mouse anti-human B7-1/CD80 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0707-CA Mouse anti-human B7-1/CD80 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0707-DA Biotinylated goat anti-human B7-1/CD80 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) EZ0707-DA Biotinylated goat anti-human B7-1/CD80 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ0707-ST Lyophilized recombinant human B7-1/CD80 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. EZ0707-ST Lyophilized recombinant human B7-1/CD80 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit.
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 33048 MW
- Specificity
- Natural and recombinant human CD80
- Gene Name Synonym
- T-lymphocyte activation antigen CD80
- Subcellular Location
- Membrane; Single-pass type I membrane protein.
- Sequence Similarities
- Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
- Protein Name
- T-lymphocyte activation antigen CD80
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.052 0.097 0.236 0.508 0.897 1.220 2.023 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.052 0.097 0.236 0.508 0.897 1.220 2.023 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.052 0.097 0.236 0.508 0.897 1.220 2.023 O.D. 0.008 0.052 0.097 0.236 0.508 0.897 1.220 2.023
- Material Required But Not Provided
- 1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
