- Catalog number
- EZ0370
- Size / count
- 5 Plates/Kit
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1) . ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1
- Synonyms
- Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD54; ICAM1
- Gene Name
- ICAM1
- Gene ID
- 3383
- UniProt
- P05362
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q28-E480
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Atherosclerosis, Cardiovascular, Cell Adhesion Molecules, Cell Type Markers, Hematopoietic Progenitors, Immunology, Lymphoid, Stem Cells, T Lymphocytic Lineage, Vascular Inflammation
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Function
- ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) . During leukocyte trans- endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. .
- Components
- Catalog number Description Quantity Storage of opened/reconstituted material EZ0370-CA Mouse anti-human ICAM-1/CD54 polyclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0370-DA Biotinylated sheep anti-human ICAM-1/CD54 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ0370-ST Lyophilized recombinant human ICAM-1/CD54 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0370-CA Mouse anti-human ICAM-1/CD54 polyclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0370-DA Biotinylated sheep anti-human ICAM-1/CD54 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ0370-ST Lyophilized recombinant human ICAM-1/CD54 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0370-CA Mouse anti-human ICAM-1/CD54 polyclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0370-CA Mouse anti-human ICAM-1/CD54 polyclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0370-DA Biotinylated sheep anti-human ICAM-1/CD54 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) EZ0370-DA Biotinylated sheep anti-human ICAM-1/CD54 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ0370-ST Lyophilized recombinant human ICAM-1/CD54 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. EZ0370-ST Lyophilized recombinant human ICAM-1/CD54 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit.
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 57825 MW
- Specificity
- Natural and recombinant human sICAM-1
- Gene Name Synonym
- Intercellular adhesion molecule 1
- Subcellular Location
- Membrane; Single-pass type I membrane protein.
- Sequence Similarities
- Belongs to the immunoglobulin superfamily. ICAM family.
- Protein Name
- Intercellular adhesion molecule 1
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.001 0.058 0.106 0.208 0.526 1.025 1.503 2.098 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.001 0.058 0.106 0.208 0.526 1.025 1.503 2.098 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.001 0.058 0.106 0.208 0.526 1.025 1.503 2.098 O.D. 0.001 0.058 0.106 0.208 0.526 1.025 1.503 2.098
- Material Required But Not Provided
- 1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
