- Catalog number
- EZ1209
- Size / count
- 5 Plates/Kit
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Amyloid P component, serum (SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component (AP), a 25kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called amyloid. It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.
- Synonyms
- Serum amyloid P-component; SAP; Apcs; Ptx2, Sap
- Gene Name
- APCS
- Gene ID
- 29339
- UniProt
- P23680
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q21-S228
- Applications
- ELISA
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Components
- Catalog number Description Quantity Storage of opened/reconstituted material EZ1209-CA Mouse anti-rat SAP/PTX2/APCS monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1209-DA Biotinylated goat anti-rat SAP/PTX2/APCS polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ1209-ST Lyophilized recombinant rat SAP/PTX2/APCS standard 100 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ1209-CA Mouse anti-rat SAP/PTX2/APCS monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1209-DA Biotinylated goat anti-rat SAP/PTX2/APCS polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ1209-ST Lyophilized recombinant rat SAP/PTX2/APCS standard 100 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ1209-CA Mouse anti-rat SAP/PTX2/APCS monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1209-CA Mouse anti-rat SAP/PTX2/APCS monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1209-DA Biotinylated goat anti-rat SAP/PTX2/APCS polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) EZ1209-DA Biotinylated goat anti-rat SAP/PTX2/APCS polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ1209-ST Lyophilized recombinant rat SAP/PTX2/APCS standard 100 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. EZ1209-ST Lyophilized recombinant rat SAP/PTX2/APCS standard 100 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit.
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 26176 MW
- Specificity
- Natural and recombinant rat SAP
- Gene Name Synonym
- Serum amyloid P-component
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the pentaxin family.
- Protein Name
- Serum amyloid P-component
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.007 0.168 0.271 0.454 0.859 1.286 1.790 2.130 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.007 0.168 0.271 0.454 0.859 1.286 1.790 2.130 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.007 0.168 0.271 0.454 0.859 1.286 1.790 2.130 O.D. 0.007 0.168 0.271 0.454 0.859 1.286 1.790 2.130
- Material Required But Not Provided
- 1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
