Mouse IL-2/Interleukin-2 PicoKine® Quick ELISA Kit

About this product

Mouse IL-2/Interleukin-2 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests) . Quantitate Mouse Il2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0398

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2 (IL2) is assigned to chromosome 4. Mouse IL-2 contains 149 amino acids in mature form with the molecular mass of 17.2KDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 40 36 39 38 1.58 4.1% Sample 2 156 162 166 155 159 4.49 2.8% Sample 3 467 448 470 463 462 8.45 1.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 40 36 39 38 1.58 4.1% Sample 1 37 40 36 39 38 1.58 4.1% Sample 2 156 162 166 155 159 4.49 2.8% Sample 2 156 162 166 155 159 4.49 2.8% Sample 3 467 448 470 463 462 8.45 1.8% Sample 3 467 448 470 463 462 8.45 1.8%

Synonyms

Interleukin-2; IL-2; T-cell growth factor; TCGF; Il2; Il-2

Gene Name

Il2

Gene ID

16183

UniProt

P04351

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A21-Q169

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 156 467 40 166 452 Standard deviation 2.1 8.58 37.7%5 2.6 10.62 37.06 CV (%) 5.7% 5.5% 7.7% 6.5% 6.4% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 156 467 40 166 452 Standard deviation 2.1 8.58 37.7%5 2.6 10.62 37.06 CV (%) 5.7% 5.5% 7.7% 6.5% 6.4% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 37 156 467 40 166 452 Mean (pg/ml) 37 156 467 40 166 452 Standard deviation 2.1 8.58 37.7%5 2.6 10.62 37.06 Standard deviation 2.1 8.58 37.7%5 2.6 10.62 37.06 CV (%) 5.7% 5.5% 7.7% 6.5% 6.4% 8.2% CV (%) 5.7% 5.5% 7.7% 6.5% 6.4% 8.2%

Sensitivity

<1 pg/mL

Function

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells.

Components

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

19400 MW

Specificity

Natural and recombinant mouse IL-2

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Interleukin-2

Subcellular Location

Secreted.

Protein Name

Interleukin-2

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.102 0.200 0.399 0.634 1.357 1.726 2.042 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.102 0.200 0.399 0.634 1.357 1.726 2.042 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.102 0.200 0.399 0.634 1.357 1.726 2.042 O.D. 0.033 0.102 0.200 0.399 0.634 1.357 1.726 2.042

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.