- Catalog number
- FEK0364
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts ly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 30 31 32 30 1.47 4.9% Sample 2 213 209 210 221 213 4.71 2.2% Sample 3 388 374 388 433 395 22.25 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 30 31 32 30 1.47 4.9% Sample 1 28 30 31 32 30 1.47 4.9% Sample 2 213 209 210 221 213 4.71 2.2% Sample 2 213 209 210 221 213 4.71 2.2% Sample 3 388 374 388 433 395 22.25 5.6% Sample 3 388 374 388 433 395 22.25 5.6%
- Synonyms
- Granulocyte-macrophage colony-stimulating factor; GM-CSF; Colony-stimulating factor; CSF; Molgramostin; Sargramostim; CSF2; GMCSF
- Gene Name
- CSF2
- Gene ID
- 1437
- UniProt
- P04141
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: A18-E144
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 213 388 27 219 407 Standard deviation 1.34 15.76 19.78 1.8 20.14 26.04 CV (%) 4.8% 7.4% 6.4% 6.7% 9.2% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 213 388 27 219 407 Standard deviation 1.34 15.76 19.78 1.8 20.14 26.04 CV (%) 4.8% 7.4% 6.4% 6.7% 9.2% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 28 213 388 27 219 407 Mean (pg/ml) 28 213 388 27 219 407 Standard deviation 1.34 15.76 19.78 1.8 20.14 26.04 Standard deviation 1.34 15.76 19.78 1.8 20.14 26.04 CV (%) 4.8% 7.4% 6.4% 6.7% 9.2% 6.4% CV (%) 4.8% 7.4% 6.4% 6.7% 9.2% 6.4%
- Sensitivity
- <1 pg/mL
- Function
- Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
- Components
- Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Calculated Molecular Weight
- 16295 MW
- Specificity
- Natural and recombinant human CSF2
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Granulocyte-macrophage colony-stimulating factor
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the GM-CSF family.
- Protein Name
- Granulocyte-macrophage colony-stimulating factor
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.022 0.233 0.439 0.799 1.215 1.432 1.736 2.016 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.022 0.233 0.439 0.799 1.215 1.432 1.736 2.016 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.022 0.233 0.439 0.799 1.215 1.432 1.736 2.016 O.D. 0.022 0.233 0.439 0.799 1.215 1.432 1.736 2.016
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
